Product Name
ERCC8, siRNA
Full Product Name
ERCC8 siRNA (Human)
Product Synonym Names
CKN1; CSA; DNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA
Product Gene Name
ERCC8 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13216
Specificity
ERCC8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ERCC8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of ERCC8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
ERCC8 sirna
siRNA to inhibit ERCC8 expression using RNA interference
Applications Tested/Suitable for ERCC8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for ERCC8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000073.1
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NCBI GenBank Nucleotide #
NM_000082.3
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UniProt Primary Accession #
Q13216
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UniProt Secondary Accession #
Q6FHX5; Q96GB9; B2RB64[Other Products]
UniProt Related Accession #
Q13216[Other Products]
Molecular Weight
23,182 Da
NCBI Official Full Name
DNA excision repair protein ERCC-8 isoform 1
NCBI Official Synonym Full Names
excision repair cross-complementation group 8
NCBI Official Symbol
ERCC8??[Similar Products]
NCBI Official Synonym Symbols
CSA; CKN1; UVSS2
??[Similar Products]
NCBI Protein Information
DNA excision repair protein ERCC-8
UniProt Protein Name
DNA excision repair protein ERCC-8
UniProt Synonym Protein Names
Cockayne syndrome WD repeat protein CSA
Protein Family
DNA excision repair protein
UniProt Gene Name
ERCC8??[Similar Products]
UniProt Synonym Gene Names
CKN1; CSA??[Similar Products]
UniProt Entry Name
ERCC8_HUMAN
NCBI Summary for ERCC8
This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
UniProt Comments for ERCC8
ERCC8: Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription- coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Defects in ERCC8 are the cause of Cockayne syndrome type A (CSA). Cockayne syndrome is a rare disorder characterized by cutaneous sensitivity to sunlight, abnormal and slow growth, cachectic dwarfism, progeroid appearance, progressive pigmentary retinopathy and sensorineural deafness. There is delayed neural development and severe progressive neurologic degeneration resulting in mental retardation. Two clinical forms are recognized: in the classical form or Cockayne syndrome type 1, the symptoms are progressive and typically become apparent within the first few years or life; the less common Cockayne syndrome type 2 is characterized by more severe symptoms that manifest prenatally. Cockayne syndrome shows some overlap with certain forms of xeroderma pigmentosum. Unlike xeroderma pigmentosum, patients with Cockayne syndrome do not manifest increased freckling and other pigmentation abnormalities in the skin and have no significant increase in skin cancer. Defects in ERCC8 are the cause of UV-sensitive syndrome type 2 (UVSS2). An autosomal recessive disorder characterized by cutaneous photosensitivity and mild freckling in the absence of neurological abnormalities or skin tumors. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA repair, damage; Helicase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q12.1
Cellular Component: nucleoplasm; nucleotide-excision repair complex; protein complex; nuclear matrix; nucleus
Molecular Function: DNA-dependent ATPase activity; protein binding; DNA helicase activity; protein complex binding; ubiquitin-protein ligase activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; protein autoubiquitination; positive regulation of DNA repair; protein polyubiquitination; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; response to oxidative stress; DNA repair; response to DNA damage stimulus; response to UV; response to X-ray
Disease: Cockayne Syndrome A; Uv-sensitive Syndrome 2
Research Articles on ERCC8
1. A novel function of Cockayne syndrome A protein as transcription factor of RNA polymerase I in the nucleolus is shown.
Precautions
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Disclaimer
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