CP24;CYP24;HCAI;HCINF1;P450-CC24

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Affinity purification

Store at -20?. Avoid freeze / thaw cycles.

Small volumes of anti-CYP24A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene

Polyclonal

Western Blot (WB), Immunohistochemistry (IHC)

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Western blot analysis of extracts of various cell lines, using CYP24A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat lung using CYP24A1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat kidney using CYP24A1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using CYP24A1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CYP24A1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using CYP24A1 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using CYP24A1 antibody at dilution of 1:200 (40x lens).

59kDa

cytochrome P450, family 24, subfamily A, polypeptide 1

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase??[Similar Products]

CP24A_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.126; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: mitochondrial inner membrane

Molecular Function: iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: osteoblast differentiation; steroid metabolic process; response to vitamin D; vitamin metabolic process; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process

Disease: Hypercalcemia, Infantile

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