Anti-CYP24 / CYP24A1 Antibody (Internal) IHC-plus; CYP24A1; 24-OHase; CP24; CYP24; Cytochrome P450-CC24; Exo-mitochondrial protein; HCAI; p450-CC24; Vitamin D 24-hydroxylase; Cytochrome P450 24A1; Vitamin D(3) 24-hydroxylase; Human CYP24; CYP24A1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Goat

Human CYP24A1 / CYP24. This antibody is expected to recognize both reported isoforms (NP_000773.2; NP_001122387.1).

Immunoaffinity Purified

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

0.5 mg/ml (lot specific)

Store at -20 degree C. Minimize freezing and thawing.

Small volumes of anti-CYP24 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Family: Cytochrome P450

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

ELISA (1:32000), IHC-P (3.75 ug/ml), WB (0.1 - 0.3 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 50kD band observed in Human and Rat Liver lysates (calculated MW of 51.2kD according to NP_001122387.1). Recommended concentration: 0.1-0.3 ug/ml.

Anti-CYP24 / CYP24A1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

CYP24A1 antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

43,051 Da

cytochrome P450, family 24, subfamily A, polypeptide 1

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase)

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase??[Similar Products]

CP24A_HUMAN

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; EC 1.14.13.126

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: mitochondrial inner membrane

Molecular Function: iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity

Biological Process: osteoblast differentiation; steroid metabolic process; vitamin metabolic process; response to vitamin D; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process

Disease: Hypercalcemia, Infantile

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