Rabbit Polyclonal to Human DEPDC5; Human DEPDC5; DEP domain containing 5; DEP.5; KIAA0645

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Human DEPDC5

Immunoaffinity purified

PBS, 0.09% sodium azide

Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.

Small volumes of anti-DEPDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

DEPDC5 Antibody, DEP domain containing 5 Antibody, DEP.5 Antibody, KIAA0645 Antibody Description: DEPDC5 is a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci.

Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)

Flow Cytometry (1:10 - 1:50)
IHC-P (5 ug/ml)
WB (1:1000)

Anti-DEPDC5 antibody IHC staining of human lung, alveoli and macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot of DEPD5 Antibody antibody pre-incubated without(lane 1) and with(lane 2) blocking peptide in K562 cell line lysate. DEPD5 Antibody (arrow) was detected using the purified antibody.

DEPD5 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

177,910 Da

DEP domain containing 5

DEP domain-containing protein 5

DEP domain-containing protein 5

KIAA0645??[Similar Products]

DEPD5_HUMAN

This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: cytosol; lysosomal membrane; lysosome; perinuclear region of cytoplasm

Molecular Function: GTPase activator activity; protein complex binding

Biological Process: cellular response to amino acid starvation; negative regulation of TOR signaling pathway; positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci

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