Full Product Name
RUNX2 Antibody
Product Synonym Names
Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; RUNX2; AML3; CBFA1; OSF2; PEBP2A
Product Gene Name
anti-RUNX2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q13950
Purity/Purification
>95%
Protein G Purified
Immunogen
Recombinant Human Runt-related transcription factor 2 protein (219-404AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of anti-RUNX2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-RUNX2 antibody
Neuroscience
Applications Tested/Suitable for anti-RUNX2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-RUNX2 antibody
WB: 1:500-1:5000
Western Blot (WB) of anti-RUNX2 antibody
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate
All lanes: RUNX2 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 57, 56, 55 kDa
Observed band size: 57 kDa
NCBI/Uniprot data below describe general gene information for RUNX2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001015051.3
[Other Products]
NCBI GenBank Nucleotide #
NM_001015051.3
[Other Products]
UniProt Primary Accession #
Q13950
[Other Products]
UniProt Secondary Accession #
O14614; O14615; O95181[Other Products]
UniProt Related Accession #
Q13950[Other Products]
Molecular Weight
55,054 Da
NCBI Official Full Name
runt-related transcription factor 2 isoform b
NCBI Official Synonym Full Names
runt related transcription factor 2
NCBI Official Symbol
RUNX2??[Similar Products]
NCBI Official Synonym Symbols
CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
??[Similar Products]
NCBI Protein Information
runt-related transcription factor 2
UniProt Protein Name
Runt-related transcription factor 2
UniProt Synonym Protein Names
Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
Protein Family
Runt-related transcription factor
UniProt Gene Name
RUNX2??[Similar Products]
UniProt Synonym Gene Names
AML3; CBFA1; OSF2; PEBP2A; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A??[Similar Products]
NCBI Summary for RUNX2
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]
UniProt Comments for RUNX2
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed:28505335, PubMed:28738062, PubMed:28703881). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (). Inhibits KAT6B-dependent transcriptional activation.
Research Articles on RUNX2
1. All these findings indicate that RUNX2 mutation can reduce the osteogenic capacity of dental follicle cells through inhibiting osteoblast-associated genes, thereby disturbing alveolar bone formation, which serves as a motive force for tooth eruption
Precautions
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Disclaimer
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