Product Name
Antithrombin (AT), Recombinant Protein
Full Product Name
Recombinant Antithrombin (AT)
Product Gene Name
AT recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-HGNPVD D ICIAKPRDIP VNPLCIYRSP GKKATEEDGS EQKVPEATNR RVWELSKANS RFATNFYQHL ADSKNDNDNI FLSPLSISTA FAMTKLGACN DTLKQLMEVF KFDTISEKTS DQIHFFFAKL NCRLYRKANK SSDLVSANRL FGDKSLTFNE SYQDVSEVVY GAKLQPLDFK ENPEQSRVTI NNWVANKTEG RIKDVIPQGA INELTALVLV NTIYFKGLWK SKFSPENTRK EPFYKVDGQS CPVPMMYQEG KFKYRRVAEG TQVLELPFKG DDITMVLILP KPEKSLAKVE QELTPELLQE WLDELSETML VVHMPRFRTE DGFSLKEQLQ DMGLIDLFSP EKSQLPGIVA GGRDDLYVSD AFHKAFLEVN EEGSEAAAST SVVITGRSLN PNRVTFKANR PFLVLIREVA LNTIIFMGRV ANPCVN
Chromosome Location
Chromosome: 1; NC_000067.6 (160978606..161003015). Location: 1 H2.1; 1 69.75 cM
3D Structure
ModBase 3D Structure for P32261
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
His33-~Asn465 (Accession # P32261) with N-terminal His-Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.
Other Notes
Small volumes of AT recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
AT recombinant protein
About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
Applications Tested/Suitable for AT recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
SDS-Page of AT recombinant protein
NCBI/Uniprot data below describe general gene information for AT. It may not necessarily be applicable to this product.
NCBI Accession #
NP_543120.1
[Other Products]
NCBI GenBank Nucleotide #
NM_080844.4
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UniProt Primary Accession #
P32261
[Other Products]
UniProt Related Accession #
P32261[Other Products]
NCBI Official Full Name
antithrombin-III
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1
NCBI Official Symbol
Serpinc1??[Similar Products]
NCBI Official Synonym Symbols
At3; At-3; ATIII; AI114908
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NCBI Protein Information
antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1
UniProt Protein Name
Antithrombin-III
UniProt Synonym Protein Names
Serpin C1
UniProt Gene Name
Serpinc1??[Similar Products]
UniProt Synonym Gene Names
At3; ATIII??[Similar Products]
UniProt Entry Name
ANT3_MOUSE
UniProt Comments for AT
SERPINC1: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D). AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin- binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. Belongs to the serpin family.
Protein type: Inhibitor; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; serine-type endopeptidase inhibitor activity; protease binding; protease inhibitor activity
Biological Process: hemostasis; negative regulation of inflammatory response; negative regulation of peptidase activity; blood coagulation; response to nutrient
Research Articles on AT
1. RNA interference of Serpinc1 and/or Proc allows for evaluation of the function of these genes in vivo and provides a novel, controlled mouse model for spontaneous venous thrombosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
