Full Product Name
CBFA1 antibody
Product Synonym Names
Polyclonal CBFA1; Anti-CBFA1; CBFA 1; Core binding factor A1; CBFA-1; RUNX2; CBFA1
Product Gene Name
anti-CBFA1 antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Purity/Purification
CBFA1 antibody was purified by affinity chromatography
Form/Format
Supplied in lyophilized form
Biological Significance
CBFA1 is a runt-like transcription factor essential for osteoblast differentiation. This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. D'Souza et al.(1999) indicate a non-redundant role for Cbfa1 in tooth development that may be distinct from that in bone formation. In odontogenesis, Cbfa1 is not involved in the early signaling networks regulating tooth initiation and early morphogenesis but regulates key epithelial-mesenchymal interactions that control advancing morphogenesis and histodifferentiation of the epithelial enamel organ.
Immunogen
CBFA1 antibody was raised in Rabbit using a synthetic peptide corresponding to a sequence in the middle region of human CBFA1, identical to the related rat and Rabbit sequence as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage aliquot and store at -20 degree C, avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-CBFA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CBFA1 antibody
Affinity purified Rabbit polyclonal CBFA1 antibody
Product Categories/Family for anti-CBFA1 antibody
DNA & RNA; Purified Polyclonal Antibodies
Applications Tested/Suitable for anti-CBFA1 antibody
Western Blot (WB)
Application Notes for anti-CBFA1 antibody
Optimal conditions to be determined by the end user
Western Blot (WB) of anti-CBFA1 antibody
Lane 1: Rat Thymus Tissue Lysate
NCBI/Uniprot data below describe general gene information for CBFA1. It may not necessarily be applicable to this product.
NCBI Accession #
BAA85346.1
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UniProt Secondary Accession #
O35183; Q08776; Q9JLN0; Q9QUQ6; Q9QY29; Q9R0U4; Q9Z2J7[Other Products]
UniProt Related Accession #
Q08775[Other Products]
Molecular Weight
52,285 Da
NCBI Official Full Name
Cbfa1, partial
NCBI Official Synonym Full Names
runt related transcription factor 2
NCBI Official Symbol
Runx2??[Similar Products]
NCBI Official Synonym Symbols
Cbf; LS3; AML3; Osf2; Cbfa1; Cbfa-1; PEBP2aA; Pebp2a1; Pebpa2a
??[Similar Products]
NCBI Protein Information
runt-related transcription factor 2
UniProt Protein Name
Runt-related transcription factor 2
UniProt Synonym Protein Names
Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific transcription factor 2; OSF-2; Polyomavirus enhancer-binding protein 2 alpha A subunit; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit
UniProt Gene Name
Runx2??[Similar Products]
UniProt Synonym Gene Names
Aml3; Cbfa1; Osf2; Pebp2a; CBF-alpha-1; OSF-2; PEA2-alpha A; PEBP2-alpha A??[Similar Products]
UniProt Entry Name
RUNX2_MOUSE
UniProt Comments for CBFA1
AML3: Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'- PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Inhibits KAT6B-dependent transcriptional activation. Interaction with SATB2 results in enhanced DNA binding and transactivation by these transcription factors. Heterodimer of an alpha and a beta subunit. Interacts with HIVEP3 and HIPK3. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with XRCC6 (Ku70) and XRCC5 (Ku80). Interacts with KAT6A and KAT6B. Binds to cyclin B1 CCNB1. Interacts with DDX5. Specifically expressed in osteoblasts. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; cytoplasm; nucleus
Molecular Function: protein domain specific binding; protein binding; DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor activity; transcription factor binding; ATP binding
Biological Process: transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; positive regulation of transcription, DNA-dependent; cell maturation; BMP signaling pathway; neuron differentiation; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; chondrocyte differentiation; skeletal development; endochondral ossification; T cell differentiation; regulation of odontogenesis of dentine-containing teeth; transcription, DNA-dependent; regulation of osteoblast differentiation; regulation of fibroblast growth factor receptor signaling pathway; chondrocyte development; stem cell differentiation; embryonic cranial skeleton morphogenesis; positive regulation of ossification; odontogenesis of dentine-containing teeth; osteoblast development; osteoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of chondrocyte differentiation; skeletal morphogenesis; negative regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter; regulation of ossification; negative regulation of transcription, DNA-dependent; osteoblast fate commitment
Research Articles on CBFA1
1. TNF-alpha/IL-1beta- and BMP-2-activated p38 and ERK1/2 signaling have opposing roles that converge on Runx2 to regulate osteoblastic differentiation.
Precautions
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