ED1; HED; EDA1; EDA2; ODT1; XHED; XLHED; ED1-A1; ED1-A2; STHAGX1

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

IgG

Rabbit

Affinity Purification

1mg/ml (lot specific)

Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Small volumes of anti-EDA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene.

Polyclonal

Western Blot (WB)

WB: 1:500 - 1:2000

Western blot analysis of extracts of various cell lines, using EDA antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human uterine cancer using EDA Antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat liver using EDA Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat pancreas using EDA Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using EDA Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse skeletal muscle using EDA Antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using EDA Antibody at dilution of 1:100 (40x lens).

391

ectodysplasin A

ectodysplasin-A; oligodontia 1; X-linked anhidroitic ectodermal dysplasia protein

Ectodysplasin-A

ED1; EDA2; EDA protein??[Similar Products]

EDA_HUMAN

The protein encoded by this gene is a type II membrane protein that can be cleaved by furin to produce a secreted form. The encoded protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in this gene are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia. Several transcript variants encoding many different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

EDA: Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR. Defects in EDA are the cause of ectodermal dysplasia type 1 (ED1); also known as Christ-Siemens-Touraine syndrome or X-linked hypohidrotic ectodermal dysplasia (XLHED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. ED1 is a disease characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. ED1 is the most common form of over 150 clinically distinct ectodermal dysplasias. Defects in EDA are the cause of tooth agenesis selective X-linked type 1 (STHAGX1). A form of selective tooth agenesis, a common anomaly characterized by the congenital absence of one or more teeth. Selective tooth agenesis without associated systemic disorders has sometimes been divided into 2 types: oligodontia, defined as agenesis of 6 or more permanent teeth, and hypodontia, defined as agenesis of less than 6 teeth. The number in both cases does not include absence of third molars (wisdom teeth). Belongs to the tumor necrosis factor family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12-q13.1

Cellular Component: endoplasmic reticulum membrane; cytoskeleton; collagen; membrane; intracellular membrane-bound organelle; apical part of cell; integral to plasma membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: pigmentation; cell-matrix adhesion; ectoderm development; immune response; positive regulation of NF-kappaB import into nucleus; gene expression; signal transduction; cell differentiation; activation of NF-kappaB transcription factor; odontogenesis of dentine-containing teeth

Disease: Tooth Agenesis, Selective, X-linked, 1; Ectodermal Dysplasia 1, Hypohidrotic, X-linked

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