Rabbit anti-SOX-2; Sry-related high-mobility group (HMG) box 2

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 3; NC_000003.11 (181429722..181432224). Location: 3q26.3-q27

Rabbit

This antibody recognizes ~35 kDa of human SOX-2 protein. It also reacts with mouse and rat. The other species are not tested.

The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.

This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer

Size: 100 ug/200 ul (lot specific)

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Small volumes of anti-SOX-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

The SOX2 is a transcription factor, a member of the SRY-related HMG-box (SOX) family involved in the regulation of embryonic development and in the determination of cell fate. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. When SOX-2 is expressed in self-renewing progenitor cells, it acts to inhibit neuronal differentiation. Sox-2 is considered as an appropriate cell-specific marker in vivo dynamics of the neural progenitor cell compartment as well as in the ***** brain.

ELISA, Western Blot, Immunohistochemistry

Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml

mouse stem cells was stained by Rabbit anti-SOX-2 at 1:250, and visualized by FITClabled anti Rabbit IgG. The nuclear staining is observed.

The whole cell lysate derived from embryonic mouse brain immunoblotted by Rabbit anti-SOX-2 at 1:500

35 kDa

SRY (sex determining region Y)-box 2

transcription factor SOX-2; OTTHUMP00000212684; transcription factor SOX2; SRY-related HMG-box gene 2

Transcription factor SOX-2

SOX2_HUMAN

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

Function: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206

By similarity. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation

By similarity. Ref.5

Subunit structure: Interacts with ZSCAN10

By similarity. Interacts with SOX3 and FGFR1

Subcellular location: Nucleus.

Post-translational modification: Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation

Involvement in disease: Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [

MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. Ref.4

Biotechnological use: POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differenciated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Ref.5

Sequence similarities: Contains 1 HMG box DNA-binding domain.

Sequence caution: The sequence AAA35997.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA83435.1 differs from that shown. Reason: Erroneous initiation.

Mila Komitova and Peter S. Eriksson. Sox-2 is expressed by neural progenitors and astroglia in the ***** rat brain Neuroscience Letters Volume 369, Issue 1, 7 October 2004, Pages 24-27

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