For Research Use Only. Not for use in diagnostic procedures.

Mouse

Store at 4 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of KLKB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS136095 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mouse Prekallikrein (KLKB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLKB1. The ELISA analytical biochemical technique of the MBS136095 kit is based on KLKB1 antibody-KLKB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLKB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLKB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Prekallikrein is the glycosylated single chain zymogen precursor of the plasma serine protease kallikrein. Plasma prekallikrein circulates with kininogen and is activated by Factor XIIa in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma prekallikrein is associated with diabetes and cardiovascular disease. The sensitive quantitative measurement of total mouse prekallikrein antigen in plasma samples is easily performed with this 96 well strip format ELISA kit. Kallikrein will be detected by the assay. The concentration of prekallirein in normal human plasma is 15-55 ug/ml. Normal values of prekallikrein in mouse plasma have not been conclusively determined but are believed to be similar to human plasma based on in-house testing. The assay measures mouse prekallikrein in the 0.1-100 ng/ml range. Samples giving mouse prekallikrein levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:10,000-1:20,000 dilution for plasma is suggested for best results. Mouse prekallikrein will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-mouse prekallikrein primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with streptavidin conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of prekallikrein in the samples. A standard calibration curve is prepared using dilutions of purified prekallikrein and is measured along with the test samples.

Mouse Prekallikrein ELISA Kits; Mouse Kits

71,383 Da

kallikrein B, plasma 1

plasma kallikrein

Plasma kallikrein

Klk3; Pk??[Similar Products]

This gene encodes a member of the kallikrein subfamily of serine proteases that are involved in diverse physiological functions such as skin desquamation, tooth enamel formation, seminal liquefaction, synaptic neural plasticity and brain function. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric enzyme comprised of heavy and light chains. A complete deletion of the encoded protein prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. [provided by RefSeq, May 2016]

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.