Full Product Name
EMX2 siRNA (Mouse)
Product Synonym Names
EMX-2; Homeobox protein EMX2; Empty spiracles homolog 2; Empty spiracles-like protein 2
Product Gene Name
EMX2 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q04744
Specificity
EMX2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse EMX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of EMX2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
EMX2 sirna
siRNA to inhibit EMX2 expression using RNA interference
Applications Tested/Suitable for EMX2 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for EMX2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_034262.2
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NCBI GenBank Nucleotide #
NM_010132.2
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UniProt Primary Accession #
Q04744
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UniProt Secondary Accession #
Q80UE7; G3X9A6[Other Products]
UniProt Related Accession #
Q04744[Other Products]
Molecular Weight
28,374 Da
NCBI Official Full Name
homeobox protein EMX2
NCBI Official Synonym Full Names
empty spiracles homeobox 2
NCBI Official Symbol
Emx2??[Similar Products]
NCBI Official Synonym Symbols
Pdo
??[Similar Products]
NCBI Protein Information
homeobox protein EMX2
UniProt Protein Name
Homeobox protein EMX2
UniProt Synonym Protein Names
Empty spiracles homolog 2; Empty spiracles-like protein 2
Protein Family
Homeobox protein
UniProt Gene Name
Emx2??[Similar Products]
UniProt Synonym Gene Names
Emx-2??[Similar Products]
UniProt Entry Name
EMX2_MOUSE
UniProt Comments for EMX2
EMX2: Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system. Defects in EMX2 are the cause of schizencephaly (SCHZC). Schizencephaly is an extremely rare human congenital disorder characterized by a full-thickness cleft within the cerebral hemispheres. These clefts are lined with gray matter and most commonly involve the parasylvian regions. Large portions of the cerebral hemispheres may be absent and replaced by cerebro- spinal fluid. Belongs to the EMX homeobox family.
Protein type: DNA-binding
Cellular Component: nucleus
Molecular Function: DNA binding; sequence-specific DNA binding
Biological Process: forebrain cell migration; anterior/posterior pattern formation; response to drug; neuron differentiation; dentate gyrus development; regulation of transcription, DNA-dependent; regulation of gene expression; cell proliferation in forebrain; multicellular organismal development; forebrain development; cerebral cortex regionalization; cerebral cortex development; brain development
Research Articles on EMX2
1. Emx2 expression led to inhibition of cell proliferation and Wnt signaling pathway both in vitro and in a gastric cancer xenograft model in vivo.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
