Full Product Name
RAX siRNA (Human)
Product Synonym Names
RX; Retinal homeobox protein Rx; Retina and anterior neural fold homeobox protein
Product Gene Name
RAX sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9Y2V3
Specificity
RAX siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of RAX sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
RAX sirna
siRNA to inhibit RAX expression using RNA interference
Applications Tested/Suitable for RAX sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for RAX. It may not necessarily be applicable to this product.
NCBI Accession #
NP_038463.2
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NCBI GenBank Nucleotide #
NM_013435.2
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UniProt Primary Accession #
Q9Y2V3
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UniProt Secondary Accession #
Q86V11[Other Products]
UniProt Related Accession #
Q9Y2V3[Other Products]
Molecular Weight
10,651 Da
NCBI Official Full Name
retinal homeobox protein Rx
NCBI Official Synonym Full Names
retina and anterior neural fold homeobox
NCBI Official Symbol
RAX??[Similar Products]
NCBI Official Synonym Symbols
RX; MCOP3
??[Similar Products]
NCBI Protein Information
retinal homeobox protein Rx
UniProt Protein Name
Retinal homeobox protein Rx
UniProt Synonym Protein Names
Retina and anterior neural fold homeobox protein
Protein Family
Retinal homeobox protein
UniProt Gene Name
RAX??[Similar Products]
UniProt Synonym Gene Names
RX??[Similar Products]
UniProt Entry Name
RX_HUMAN
NCBI Summary for RAX
This gene encodes a homeobox-containing transcription factor that functions in eye development. The gene is expressed early in the eye primordia, and is required for retinal cell fate determination and also regulates stem cell proliferation. Mutations in this gene have been reported in patients with defects in ocular development, including microphthalmia, anophthalmia, and coloboma.[provided by RefSeq, Oct 2009]
UniProt Comments for RAX
RAX: Plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation. Binds to the photoreceptor conserved element-I (PCE-1/Ret 1) in the photoreceptor cell-specific arrestin promoter. Defects in RAX are the cause of microphthalmia isolated type 3 (MCOP3). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present. Belongs to the paired homeobox family. Bicoid subfamily.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 18q21.32
Cellular Component: nucleus
Biological Process: limb development; hypothalamus development; camera-type eye development; visual perception; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; pattern specification process
Disease: Microphthalmia, Isolated 3
Research Articles on RAX
1. G mutation (IVS2+3A>G) in RAX.">The three consanguineous Egyptian anophthalmia patients carry a novel homozygous c.543+3A>G mutation (IVS2+3A>G) in RAX.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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