SLC9A3; Solute Carrier Family 9 Member 3; Sodium-hydrogen antiporter 3; Na(+)/H(+) exchanger 3

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Sodium/Hydrogen Exchanger 3 (NHE3).
No significant cross-reactivity or interference between Sodium/Hydrogen Exchanger 3 (NHE3) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customera??s specifications, please inquire.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of NHE3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2000139 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Sodium/Hydrogen Exchanger 3 (NHE3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NHE3. The ELISA analytical biochemical technique of the MBS2000139 kit is based on NHE3 antibody-NHE3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NHE3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NHE3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 3 (NHE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 3 (NHE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 3 (NHE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Hydrogen Exchanger 3 (NHE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Signal transduction

91,904 Da

solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3

sodium/hydrogen exchanger 3

Sodium/hydrogen exchanger 3

NHE-3??[Similar Products]

SL9A3_HUMAN

NHE3: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Binds SLC9A3R1 and SLC9A3R2. Interacts with SHANK2. Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

Protein type: Transporter; Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cell surface; brush border membrane; apical plasma membrane; integral to membrane; plasma membrane; brush border

Molecular Function: protein binding; sodium:hydrogen antiporter activity; PDZ domain binding

Biological Process: regulation of pH; ion transport; transmembrane transport

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