Product Name
Tubulin beta-2B chain (TUBB2b), Monoclonal Antibody
Popular Item
Full Product Name
Mouse Anti Human Tubulin beta-2B chain
Product Synonym Names
TUBB2B Antibody; Tubulin beta-2B chain, Mouse Anti Human; Tubulin beta-2B chain; TUBB2B; MGC8685; FLJ98847; bA506K6.1; DKFZp566F223; RP11-506K6.1
Product Gene Name
anti-TUBB2b antibody
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Isotype
IgG2a heavy chain and k light chain
Purity/Purification
TUBB2b antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sterile Filtered clear solution.
Immunogen
Anti-human TUBB2b mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human TUBB2b amino acids 1-445 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
Other Notes
Small volumes of anti-TUBB2b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TUBB2b antibody
Introduction: TUBB2B is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. Numerous beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2b is mostly expressed in developing neurons with a dominant expression during the critical steps of corticogenesis. In addition, TUBB2B is a new critical gene involved in neuronal migration. TUBB2B is present in the nuclei and nucleoplasm, Defects in the TUBB2B gene are a cause of asymmetric polymicrogyria.
Product Categories/Family for anti-TUBB2b antibody
MONOCLONAL/POLYCLONAL ANTIBODIES, MISCELLANEOUS ANTIBODIES; Monoclonal Antibodies
Applications Tested/Suitable for anti-TUBB2b antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-TUBB2b antibody
TUBB2b antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:250.
NCBI/Uniprot data below describe general gene information for TUBB2b. It may not necessarily be applicable to this product.
NCBI Accession #
NP_821080.1
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NCBI GenBank Nucleotide #
NM_178012.4
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UniProt Secondary Accession #
A8K068[Other Products]
UniProt Related Accession #
Q9BVA1[Other Products]
Molecular Weight
49,953 Da
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin, beta 2B class IIb
NCBI Official Symbol
TUBB2B??[Similar Products]
NCBI Official Synonym Symbols
PMGYSA; bA506K6.1
??[Similar Products]
NCBI Protein Information
tubulin beta-2B chain; class II beta-tubulin isotype; class IIb beta-tubulin; tubulin, beta polypeptide paralog
UniProt Protein Name
Tubulin beta-2B chain
UniProt Gene Name
TUBB2B??[Similar Products]
UniProt Entry Name
TBB2B_HUMAN
NCBI Summary for TUBB2b
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]
UniProt Comments for TUBB2b
TUBB2B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB2B is implicated in neuronal migration. Defects in TUBB2B are a cause of polymicrogyria asymmetric (PMGA). PMGA is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination. Belongs to the tubulin family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 6p25
Cellular Component: microtubule; cytoplasm; nucleus
Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton
Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; neuron migration; transmembrane transport; microtubule-based process
Disease: Polymicrogyria, Symmetric Or Asymmetric
Research Articles on TUBB2b
1. The present study confirms that mutations in tubulin genes are responsible for complex brain malformation.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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