Product Name
Plasma Kallikrein (KLKB1), ELISA Kit
Full Product Name
Goat Plasma Kallikrein (KLKB1) ELISA Kit
Product Gene Name
KLKB1 elisa kit
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Request for Current Manual Insert
Request Current Manual
3D Structure
ModBase 3D Structure for P03952
Reproducibility
Intra-Assay: CV<10%
Inter-Assay: CV<15%
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Sample Preparation
We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.
Other Notes
Small volumes of KLKB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for KLKB1 purchase
MBS7259691 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Plasma Kallikrein (KLKB1) ELISA Kit target analytes in
biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLKB1. The ELISA analytical biochemical technique of the MBS7259691 kit is based on KLKB1 antibody-KLKB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLKB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLKB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Related Product Information for
KLKB1 elisa kit
This ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the targets.
Product Categories/Family for KLKB1 elisa kit
Cell Biology
NCBI/Uniprot data below describe general gene information for KLKB1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000883.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000892.5
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UniProt Primary Accession #
P03952
[Other Products]
UniProt Secondary Accession #
Q17RE8; Q17RE9; Q4W5C3; A6NH96; B2R8H9[Other Products]
UniProt Related Accession #
P03952[Other Products]
Molecular Weight
71,370 Da
NCBI Official Full Name
plasma kallikrein isoform 1 preproprotein
NCBI Official Synonym Full Names
kallikrein B1
NCBI Official Symbol
KLKB1??[Similar Products]
NCBI Official Synonym Symbols
PKK; PPK; KLK3; PKKD
??[Similar Products]
NCBI Protein Information
plasma kallikrein
UniProt Protein Name
Plasma kallikrein
UniProt Synonym Protein Names
Fletcher factor; Kininogenin; Plasma prekallikrein; PKK
Protein Family
Plasma kallikrein
UniProt Gene Name
KLKB1??[Similar Products]
UniProt Synonym Gene Names
KLK3; PKK??[Similar Products]
NCBI Summary for KLKB1
This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
UniProt Comments for KLKB1
The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Research Articles on KLKB1
1. We further define the interactions of keratinocyte PKK with TP63 and NF-kappaB signaling, highlighting the importance of this protein as a tumor suppressor in skin squamous cell carcinoma development.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.