For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of Human KLKB1. No significant cross-reactivity or interference between Human KLKB1 and analogues was observed.

Store at 2-8 degree C.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of KLKB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281175 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Plasma kallikrein (KLKB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLKB1. The ELISA analytical biochemical technique of the MBS281175 kit is based on KLKB1 antibody-KLKB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLKB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLKB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KLKB1 in samples. An antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLKB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

71,383 Da

kallikrein B, plasma 1

plasma kallikrein; kininogenin; fletcher factor; kallikrein 3, plasma; plasma prekallikrein; antigen, prostate specific

Plasma kallikrein

Klk3; Pk??[Similar Products]

KLKB1_MOUSE

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: EC 3.4.21.34; Protease; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: fibrinolysis; hemostasis; positive regulation of fibrinolysis; proteolysis; inflammatory response; blood coagulation; plasminogen activation

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