Product Name
PQBP1, siRNA
Full Product Name
PQBP1 siRNA (Human)
Product Synonym Names
NPW38; Polyglutamine-binding protein 1; PQBP-1; 38 kDa nuclear protein containing a WW domain; Npw38; Polyglutamine tract-binding protein 1
Product Gene Name
PQBP1 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for O60828
Specificity
PQBP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PQBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PQBP1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PQBP1 sirna
siRNA to inhibit PQBP1 expression using RNA interference
Applications Tested/Suitable for PQBP1 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PQBP1. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001027553.1
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NCBI GenBank Nucleotide #
NM_001032381.1
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UniProt Primary Accession #
O60828
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UniProt Secondary Accession #
Q4VY25; Q4VY26; Q4VY27; Q4VY29; Q4VY30; Q4VY34; Q4VY35; Q4VY36; Q4VY37; Q4VY38; Q9GZP2[Other Products]
UniProt Related Accession #
O60828[Other Products]
Molecular Weight
6,981 Da
NCBI Official Full Name
polyglutamine-binding protein 1 isoform 1
NCBI Official Synonym Full Names
polyglutamine binding protein 1
NCBI Official Symbol
PQBP1??[Similar Products]
NCBI Official Synonym Symbols
SHS; MRX2; MRX55; MRXS3; MRXS8; NPW38; RENS1
??[Similar Products]
NCBI Protein Information
polyglutamine-binding protein 1
UniProt Protein Name
Polyglutamine-binding protein 1
UniProt Synonym Protein Names
38 kDa nuclear protein containing a WW domain; Npw38; Polyglutamine tract-binding protein 1
Protein Family
Polyglutamine-binding protein
UniProt Gene Name
PQBP1??[Similar Products]
UniProt Synonym Gene Names
NPW38; PQBP-1; Npw38??[Similar Products]
UniProt Entry Name
PQBP1_HUMAN
NCBI Summary for PQBP1
This gene encodes a nuclear polyglutamine-binding protein that is involved with transcription activation. The encoded protein contains a WW domain. Mutations in this gene have been found in patients with Renpenning syndrome 1 and other syndromes with X-linked mental retardation. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.[provided by RefSeq, Nov 2009]
UniProt Comments for PQBP1
PQBP1: May suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. Can activate transcription directly or via association with the transcription machinery. May be involved in ATXN1 mutant-induced cell death. The interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit. Defects in PQBP1 are the cause of Renpenning syndrome 1 (RENS1); also known as Sutherland-Haan X-linked mental retardation syndrome (SHS) or X-linked mental retardation syndromes MRXS3/MRXS8/MRX55. The clinical features are mental retardation, microcephaly, short stature, and small testes. The craniofacies tends to be narrow and tall with upslanting palpebral fissures, abnormal nasal configuration, cupped ears, and short philtrum. The nose may appear long or bulbous, with overhanging columella. Less consistent manifestations include ocular colobomas, cardiac malformations, cleft palate, and anal anomalies. RENS1 is more frequently in males than in females where little or no expression is found. 10 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: nucleoplasm; stress granule; cytoplasm; nuclear speck; nucleus
Molecular Function: ribonucleoprotein binding; DNA binding; transcription coactivator activity
Biological Process: alternative nuclear mRNA splicing, via spliceosome; regulation of transcription, DNA-dependent; transcription, DNA-dependent; regulation of RNA splicing; regulation of dendrite morphogenesis; neurite development
Disease: Renpenning Syndrome 1
Research Articles on PQBP1
1. Study found that PQBP1 directly binds to reverse-transcribed HIV-1 DNA and interacts with cGAS to initiate an IRF3-dependent innate response.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
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