Product Name
SLC9A3, siRNA
Full Product Name
SLC9A3 siRNA (Rat)
Product Synonym Names
NHE3; Sodium/hydrogen exchanger 3; Na(+)/H(+) exchanger 3; NHE-3; Solute carrier family 9 member 3
Product Gene Name
SLC9A3 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P26433
Specificity
SLC9A3 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC9A3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC9A3 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC9A3 sirna
siRNA to inhibit SLC9A3 expression using RNA interference
Applications Tested/Suitable for SLC9A3 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC9A3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036786.1
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NCBI GenBank Nucleotide #
NM_012654.1
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UniProt Primary Accession #
P26433
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UniProt Related Accession #
P26433[Other Products]
Molecular Weight
93,105 Da
NCBI Official Full Name
sodium/hydrogen exchanger 3
NCBI Official Synonym Full Names
solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3
NCBI Official Symbol
Slc9a3??[Similar Products]
NCBI Official Synonym Symbols
Nhe3
??[Similar Products]
NCBI Protein Information
sodium/hydrogen exchanger 3
UniProt Protein Name
Sodium/hydrogen exchanger 3
UniProt Synonym Protein Names
Na(+)/H(+) exchanger 3; NHE-3; Solute carrier family 9 member 3
Protein Family
Sodium/hydrogen exchanger
UniProt Gene Name
Slc9a3??[Similar Products]
UniProt Synonym Gene Names
Nhe3; NHE-3??[Similar Products]
UniProt Entry Name
SL9A3_RAT
NCBI Summary for SLC9A3
displays Na+/H+ exchanger transport activity; mediates sodium ion transport across the renal proximal tubule [RGD, Feb 2006]
UniProt Comments for SLC9A3
NHE3: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. Binds SLC9A3R1 and SLC9A3R2. Interacts with SHANK2. Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass
Cellular Component: brush border membrane; apical plasma membrane; integral to membrane; plasma membrane; brush border
Molecular Function: protein binding; sodium:hydrogen antiporter activity; PDZ domain binding
Biological Process: circadian rhythm; receptor-mediated endocytosis; sodium ion transport; response to glucocorticoid stimulus; regulation of pH; regulation of sodium ion transport
Research Articles on SLC9A3
1. In hypertensive rats, the plasma and tissue renin angiotensin systems are activated, increased Ang II stimulates the expression of NHE3 and SGK1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
