Product Name
SLC26A9, siRNA
Full Product Name
SLC26A9 siRNA (Human)
Product Synonym Names
Solute carrier family 26 member 9; Anion transporter/exchanger protein 9
Product Gene Name
SLC26A9 sirna
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q7LBE3
Specificity
SLC26A9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SLC26A9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of SLC26A9 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
SLC26A9 sirna
siRNA to inhibit SLC26A9 expression using RNA interference
Applications Tested/Suitable for SLC26A9 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for SLC26A9. It may not necessarily be applicable to this product.
NCBI Accession #
NP_443166.1
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NCBI GenBank Nucleotide #
NM_052934.3
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UniProt Primary Accession #
Q7LBE3
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UniProt Secondary Accession #
Q96PK9; Q96RN0; A7E2V6; B1AVM8; B1AVM9; B7ZKK2[Other Products]
UniProt Related Accession #
Q7LBE3[Other Products]
Molecular Weight
97,288 Da
NCBI Official Full Name
solute carrier family 26 member 9 isoform a
NCBI Official Synonym Full Names
solute carrier family 26 (anion exchanger), member 9
NCBI Official Symbol
SLC26A9??[Similar Products]
NCBI Protein Information
solute carrier family 26 member 9
UniProt Protein Name
Solute carrier family 26 member 9
UniProt Synonym Protein Names
Anion transporter/exchanger protein 9
Protein Family
Solute carrier family
UniProt Gene Name
SLC26A9??[Similar Products]
UniProt Entry Name
S26A9_HUMAN
NCBI Summary for SLC26A9
This gene is one member of a family of sulfate/anion transporter genes. Family members are well conserved in their genomic (number and size of exons) and protein (aa length among species) structures yet have markedly different tissue expression patterns. The product of this gene is a highly selective chloride ion channel regulated by WNK kinases. Alternative splicing results in multiple transcript variants encoding differing isoforms.[provided by RefSeq, Dec 2008]
UniProt Comments for SLC26A9
SLC26A9: DIDS- and thiosulfate- sensitive anion exchanger mediating chloride, sulfate and oxalate transport. Mediates chloride/bicarbonate exchange or chloride-independent bicarbonate extrusion thus assuring bicarbonate secretion. Inhibited by ammonium and thiosulfate. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1q32.1
Cellular Component: cell surface; apical plasma membrane; plasma membrane; integral to membrane
Molecular Function: bicarbonate transmembrane transporter activity; chloride channel activity; anion:anion antiporter activity; secondary active sulfate transmembrane transporter activity; ATPase binding
Biological Process: bicarbonate transport; regulation of pH; ion transport; chloride transport; transmembrane transport; anion transport
Research Articles on SLC26A9
1. SLC26A9 single nucleotide polymorphisms modify prenatal exocrine pancreatic damage in cystic fibrosis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
