Human plasma kallikrein (KLKB1) ELISA kit; KLK3; PPK; Fletcher factor; kininogenin; plasma kallikrein B1; plasma kallikrein heavy chain; plasma kallikrein light chain; kallikrein B; plasma (Fletcher factor) 1

For Research Use Only. Not for use in diagnostic procedures.

This assay has high sensitivity and excellent specificity for detection of human KLKB1. No significant cross-reactivity or interference between human KLKB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of KLKB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS701309 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the kallikrein B, plasma (Fletcher factor) 1 (KLKB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLKB1. The ELISA analytical biochemical technique of the MBS701309 kit is based on KLKB1 antibody-KLKB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLKB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLKB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLKB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

71,370 Da

kallikrein B, plasma (Fletcher factor) 1

plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain

Plasma kallikrein

KLK3??[Similar Products]

KLKB1_HUMAN

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

Function: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Catalytic activity: Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.

Enzyme regulation: Inhibited by SERPINA5.1 PublicationManual assertion based on experiment in:Ref.9

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