Full Product Name
NHE3 Antibody: APC/Cy7
Product Synonym Names
Rabbit Anti-Rat NHE3 Polyclonal; Sodium/Hydrogen Exchanger 3 antibody; SLC9A3 Antibody; Na+/H+ exchanger 3 Antibody; isoform 3 Antibody; Solute carrier family 9 member 3 Antibody; NHE3MGC126720 Antibody; MGC126718 Antibody; solute carrier family 9 (sodium/hydrogen exchanger) member 3 Antibody; NHE-3 Antibody; Na(+)/H(+) exchanger 3 Antibody; Sodium/hydrogen exchanger 3 Antibody
Product Gene Name
anti-NHE3 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P26433
Species Reactivity
Rat, Mouse
Specificity
Detects ~84 kDa.
Purity/Purification
Protein A Purified
Form/Format
PBS, 50% Glycerol, 0.09% Sodium Azide
Concentration
1 mg/ml (lot specific)
Immunogen
Synthetic peptide mapping to AA 809 to 831 of rat sequence
Cellular Localization
Membrane
Tissue Specificity
Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level)
Certificate of Analysis
1 ug/ml was sufficient for detection of HNE3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Product Notes
Conjugated primary antibodies can be good options or alternatives to secondary antibody's for detecting a target protein. The lab manufactures a conjugated antibody from a primary parent antibody by attaching (conjugating) an available tag such as a fluorophore or protein, allowing detection of the target protein without using a secondary antibody. Conjugated primary antibodies may potentially be used to replace primary/secondary antibody pairs in various applications, such as Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry, Fluorescence-activated cell sorting, F?rster Resonance Energy Transfer (FRET). Immunocytochemistry, Immunofluorescence, Immunohistochemistry, or Western Blot. The suitability of applications for conjugates are user determined and we cannot guarantee that any antibody-conjugate combination has been tested for use in a particular application.
Please note: Conjugate tags may potentially bind in the antibody paratope, which can limit binding of the antibody to the antigen. Binding in the antibody paratope may affect the ability of the antibody to bind to the corresponding antigen (protein target) in various species and applications. There is no way to confirm or guarantee the location of the antibody tag as we cannot control the binding of the conjugate to the antibody. Researchers are responsible for evaluating antibody conjugates for the suitability of their applications.
Preparation and Storage
Store at-20 degree C.
Other Notes
Small volumes of anti-NHE3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-NHE3 antibody
Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).
Product Categories/Family for anti-NHE3 antibody
Cell Signaling; Neuroscience; Pumps/Transporters; Ion Pumps/Transporters; Cancer
Applications Tested/Suitable for anti-NHE3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-NHE3 antibody
WB: 1:1000
IHC: 1:25
Western Blot (WB) of anti-NHE3 antibody
Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:1000.
NCBI/Uniprot data below describe general gene information for NHE3. It may not necessarily be applicable to this product.
NCBI Accession #
NP_036786.1
[Other Products]
NCBI GenBank Nucleotide #
NP_036786.1
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UniProt Primary Accession #
P26433
[Other Products]
UniProt Related Accession #
P26433[Other Products]
NCBI Official Full Name
sodium/hydrogen exchanger 3
NCBI Official Synonym Full Names
solute carrier family 9 member A3
NCBI Official Symbol
Slc9a3??[Similar Products]
NCBI Official Synonym Symbols
Nhe3
??[Similar Products]
NCBI Protein Information
sodium/hydrogen exchanger 3
UniProt Protein Name
Sodium/hydrogen exchanger 3
UniProt Synonym Protein Names
Na(+)/H(+) exchanger 3; NHE-3; Solute carrier family 9 member 3
UniProt Gene Name
Slc9a3??[Similar Products]
UniProt Synonym Gene Names
Nhe3; NHE-3??[Similar Products]
NCBI Summary for NHE3
displays Na+/H+ exchanger transport activity; mediates sodium ion transport across the renal proximal tubule [RGD, Feb 2006]
UniProt Comments for NHE3
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Product References and Citations for anti-NHE3 antibody
1. Kim G.H., Ecelbarger C.A., Knepper M.A. and Packer R.K. (1999) J Am Soc Nephrol. 10: 935-942. 2. Dynia D.W., Steinmetz A.G. and Kocinsky H.S. (2010) Am J Physoil Renal Physiol. 298(3): F745-F753.
Research Articles on NHE3
1. Computational model of the renal circadian clock predicting effect of circadian clock modulation of NHE3 on proximal tubule cell on sodium transport.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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