Product Name
DUSP15, siRNA
Full Product Name
DUSP15 siRNA (Human)
Product Synonym Names
C20orf57; VHY; Dual specificity protein phosphatase 15; VH1-related member Y; Vaccinia virus VH1-related dual-specific protein phosphatase Y
Product Gene Name
DUSP15 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9H1R2
Specificity
DUSP15 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DUSP15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of DUSP15 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
DUSP15 sirna
siRNA to inhibit DUSP15 expression using RNA interference
Applications Tested/Suitable for DUSP15 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for DUSP15. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001012662.1
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NCBI GenBank Nucleotide #
NM_001012644.1
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UniProt Primary Accession #
Q9H1R2
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UniProt Secondary Accession #
Q5QP62; Q5QP63; Q5QP65; Q6PGN7; Q8N826; Q9BX24; A6NH79; A8MVC8[Other Products]
UniProt Related Accession #
Q9H1R2[Other Products]
Molecular Weight
14,721 Da
NCBI Official Full Name
dual specificity protein phosphatase 15 isoform b
NCBI Official Synonym Full Names
dual specificity phosphatase 15
NCBI Official Symbol
DUSP15??[Similar Products]
NCBI Official Synonym Symbols
VHY
??[Similar Products]
NCBI Protein Information
dual specificity protein phosphatase 15
UniProt Protein Name
Dual specificity protein phosphatase 15
UniProt Synonym Protein Names
VH1-related member Y; Vaccinia virus VH1-related dual-specific protein phosphatase Y
Protein Family
Dual specificity protein phosphatase
UniProt Gene Name
DUSP15??[Similar Products]
UniProt Synonym Gene Names
C20orf57; VHY??[Similar Products]
UniProt Entry Name
DUS15_HUMAN
NCBI Summary for DUSP15
The protein encoded by this gene belongs to the non-receptor class of the protein-tyrosine phosphatase family. The encoded protein has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for DUSP15
DUSP15: belongs to the non-receptor class of the protein-tyrosine phosphatase family. The encoded protein has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity, and therefore is known as a dual specificity phosphatase. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Protein type: Protein phosphatase, dual-specificity; Motility/polarity/chemotaxis; EC 3.1.3.48; EC 3.1.3.16
Chromosomal Location of Human Ortholog: 20q11.21
Cellular Component: cytoplasm; plasma membrane
Molecular Function: protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity
Biological Process: transforming growth factor beta receptor signaling pathway; positive regulation of JNK cascade; protein amino acid dephosphorylation; regulation of cell proliferation
Research Articles on DUSP15
1. Dusp15/VHY is a key regulator of oligodendrocyte differentiation, and PDGFR-beta and SNX6 are novel and specific Dusp15 substrates.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
