Anti -Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1131/Tyr1185) (IGF1R, IR)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000023.10 (107963179..107979643, complement). Location: Xq22.3

Monoclonal

IgG2b,k

4H346

Mouse

Recognizes phosphorylated human IGF-1R, Mr ~95kD. Also corresponds to tyrosine 1185 in human insulin receptor preprotein, containing a 27 amino acid signal peptide. Crossreacts with phosphorylated insulin and PDGF receptors which contain the I Y E motif. Slight crossreactivity detected with EGFR in A431 cells expressing very high levels of EGFR. Species Crossreactivity: mouse. Broad species crossreactivity predicted based on sequence homology. The immunizing sequence is conserved from Drosophila to mammals.

Affinity Purified
Purified by Protein G affinity chromatography.

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-IRS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Abs to Receptors

Western Blot (WB), Immunoprecipitation (IP)

Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 0.5-2ug/ml detects phosphorylated IGF-1R in RIPA lysates from stable, transfected 3T3 cells expressing the human IGF-1 receptor treated with IGF-1 and in IGFR-containing immune complexes from IGF-1 treated, but not untreated cell lysates. Lysates from untreated and IGF-1 treated 3T3 cells expressing human IGF-1 receptor were resolved by electrophoresis, transferred to nitrocellulose and probed with I7661-18A (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 5ug immunoprecipitates phosphorylated IGF-1 receptors from RIPA lysate from 3T3 cells treated with IGF-1.

133,768 Da[Similar Products]

insulin receptor substrate 4

insulin receptor substrate 4; pp160; phosphoprotein of 160 kDa; 160 kDa phosphotyrosine protein

Insulin receptor substrate 4

IRS-4; py160; pp160??[Similar Products]

IRS4_HUMAN

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq, Jul 2008]

IRS4: Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGFI signaling pathway by suppressing the function of IRS1 and IRS2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity; phosphoinositide 3-kinase binding; insulin receptor binding

Biological Process: regulation of lipid metabolic process; positive regulation of signal transduction; insulin receptor signaling pathway; signal transduction

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