Product Name
CD40-Ligand, Active Protein
Popular Item
Full Product Name
Animal Free Recombinant Human CD40-Ligand
Product Synonym Names
Animal-free Human CD40-Ligand; CD40 Ligand; TNFSF5; TRAP; CD154; gp39; T-BAM
Product Gene Name
CD40 active protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
3D Structure
ModBase 3D Structure for P29965
Purity/Purification
Purity is typically 98% as determined by HPLC, reducing and non-reducing SDS-PAGE
Form/Format
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing
10 mM sodium phosphate, pH 7.5
Bioactivity Assay
bioactive protein but no acceptaince criteria limits established
Reconstitution
Sterile water at 0.1 mg/mL
Acceptance Criteria LAL (EUs/ug)
<=1.00
Endotoxin Level (kinetic LAL analysis)
Typically less than 0.05 EUs/mug protein (50% confidence range)
Biological Activity
The activity is determined by the dose production of IL-8 by human PBMCs and is typically 5-10 ng/mL or proliferation of human PBMCs and is typically 1 mug/mL. This corresponds to expected specific activities of 1.3 x 105 units/mg or 1 x 106 units/mg respectively.
Animal Components
This product is produced with no animal-derived raw products. All processing and handling employs animal-free equipment and animal-free protocols.
Media Components
Luria Broth
Preparation and Storage
12 months from date of receipt when stored at -20 degree C to-80 degree C as supplied.
1 month when stored at 4 degree C after reconstituting as directed.
3 months when stored at -20 degree C to -80 degree C after reconstituting as directed.
Active Protein Note
Refer to the datasheet for activity details. For other activity applications, researchers should evaluate this protein for suitability in their model system.
Other Notes
Small volumes of CD40 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD40 active protein
CD40 Ligand (CD40L), or CD154, is a membrane glycoprotein and differentiation antigen that is expressed on the surface of T cells, monocytes, basophils, eosinophils, platelets, dendritic cells, and endothelial cells. CD40L binds and activates the CD40 receptor on antigen-presenting cells. CD40L stimulates B cell proliferation, immunoglobulin class switching, and antibody secretion. CD40L also induces cytokine production and tumoricidal activity in peripheral blood monocytes. CD40L is a co-stimulator of activated T cell proliferation and inflammatory protein production.
Gel Data of CD40 active protein
Biological Activity of CD40 active protein
Testing Data of CD40 active protein
NCBI/Uniprot data below describe general gene information for CD40. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000065.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000074.2
[Other Products]
UniProt Primary Accession #
P29965
[Other Products]
UniProt Related Accession #
P29965[Other Products]
Molecular Weight
Non-glycosylated protein, containing 149 amino acids, with a molecular weight of 16.3 kDa.
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
??[Similar Products]
NCBI Protein Information
CD40 ligand
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP
Protein Family
Tumor necrosis factor receptor superfamily
UniProt Gene Name
CD40LG??[Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]
NCBI Summary for CD40
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for CD40
CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xq26.3
Cellular Component: cell surface; integral to plasma membrane; plasma membrane
Molecular Function: CD40 receptor binding; protein binding
Biological Process: activation of JNK activity; activation of NF-kappaB transcription factor; B cell proliferation; inflammatory response; isotype switching; negative regulation of apoptosis; platelet activation; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-4 production; positive regulation of T cell proliferation; regulation of immune response; T cell costimulation; tumor necrosis factor-mediated signaling pathway
Disease: Immunodeficiency With Hyper-igm, Type 1
Research Articles on CD40
1. the serum levels of the soluble factors sCD40L and CXCL1 are not associated with endometriosis and are not suitable as biomarkers for disease diagnosis.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
