Product Name
CD40 Ligand/CD154/CD40L/TNFSF5 (CD40LG), Recombinant Protein
Full Product Name
Recombinant Human CD40 Ligand/CD154/CD40L/TNFSF5 Protein
Product Synonym Names
CD40LG; CD154; CD40L; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP
Product Gene Name
CD40LG recombinant protein
[Similar Products]
Product Synonym Gene Name
CD154; CD40L; TNFSF5[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL
3D Structure
ModBase 3D Structure for P29965
Purity/Purification
> 95% by SDS-PAGE.
Form/Format
Lyophilized from a 0.2 muM filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.
Endotoxin
< 1 EU/mug of the protein by LAL method.
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Protein Construction
High quality, high purity and low endotoxin recombinant Recombinant Human CD40 Ligand/CD154/CD40L/TNFSF5 Protein (RP00329), tested reactivity in Human and has been validated in SDS-PAGE.100% guaranteed.
Preparation and Storage
Store the lyophilized protein at-20 degree C to-80 degree C for long term.
After reconstitution, the protein solution is stable at-20 degree C for 3 months, at 2-8 degree C for up to 1 week.
Other Notes
Small volumes of CD40LG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
CD40LG recombinant protein
Description: Recombinant Human CD40 Ligand/CD154/CD40L/TNFSF5 Protein is produced by human cell expression system. The target protein is expressed with sequence (Met113-Leu261) of human CD40 Ligand/CD154/CD40L/TNFSF5 (Accession #P29965).
Background: This protein is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Testing Data of CD40LG recombinant protein
NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000065.1
[Other Products]
NCBI GenBank Nucleotide #
NM_000074.2
[Other Products]
UniProt Primary Accession #
P29965
[Other Products]
UniProt Related Accession #
P29965[Other Products]
Molecular Weight
29,274 Da
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
??[Similar Products]
NCBI Protein Information
CD40 ligand
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP
Protein Family
CD40 ligand
UniProt Gene Name
CD40LG??[Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]
NCBI Summary for CD40LG
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for CD40LG
Cytokine that binds to CD40/TNFRSF5 (PubMed:1280226). Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching ().
Research Articles on CD40LG
1. this study reports the clinical and CD40L genetic features of six Iranian hyper IgM syndrome patients
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
