Anti -Insulin-Like Growth Factor I Receptor/Insulin Receptor, phosphorylated (Tyr1131/Tyr1158) (IGF1R, IR)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: X; NC_000023.10 (107963179..107979643, complement). Location: Xq22.3

Polyclonal

Rabbit

Recognizes human IR/IGF1R when phosphorylated at Tyr1131 or Tyr1158. Species sequence homology: mouse and rat (100%).

Affinity Purified
Purified by immunoaffinity chromatography.

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Small volumes of anti-IRS4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Biological actions of insulin and IGF1 are mediated by their respective cell surface receptors, both of which are receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The IR and IGF1R are heterotetrameric proteins consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain. Insulin/IGF1 binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains. These differences allow insulin and IGF-1 to regulate different physiological functions through receptors that share a very similar structure. Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences. The catalytic loops within the tyrosine kinase domains of the IR/IGF1R contain a three tyrosine motif corresponding to Tyr1158, 1162 and 1163 (for the IR) and Tyr1131, 1135 and 1136 (for the IGF1R).

Antibodies; Abs to Receptors

ELISA (EL/EIA), Western Blot (WB)

Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000

133,768 Da[Similar Products]

insulin receptor substrate 4

insulin receptor substrate 4; pp160; phosphoprotein of 160 kDa; 160 kDa phosphotyrosine protein

Insulin receptor substrate 4

IRS-4; py160; pp160??[Similar Products]

IRS4_HUMAN

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.. [provided by RefSeq, Jul 2008]

IRS4: Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGFI signaling pathway by suppressing the function of IRS1 and IRS2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity; phosphoinositide 3-kinase binding; insulin receptor binding

Biological Process: regulation of lipid metabolic process; positive regulation of signal transduction; insulin receptor signaling pathway; signal transduction

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