For Research Use Only. Not for use in diagnostic procedures.

This kit recognizes natural and recombinantHumanTGFBR1. No significant cross-reactivity or interference between HumanTGFBR1 and analogues was observed.

Store at 4 degree C.

Manufactured in an ISO 9001:2015 Certified Laboratory.

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual.

Small volumes of TGFBR1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2533430 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the TGF-beta receptor type 1 (TGFBR1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TGFBR1. The ELISA analytical biochemical technique of the MBS2533430 kit is based on TGFBR1 antibody-TGFBR1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TGFBR1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TGFBR1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanTGFBR1concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGFBR1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGFBR1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGFBR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TGFBR1.You can calculate the concentration of TGFBR1in the samples by comparing the OD of the samples to the standard curve.

47,690 Da

transforming growth factor beta receptor I

TGF-beta receptor type-1

TGF-beta receptor type-1

ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I??[Similar Products]

TGFR1_HUMAN

The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

TGFBR1: a TKL kinase of the serine/threonine-protein kinase receptor (STKR) family. R1 and R2 TGF-beta receptors dimerize after binding TGF-beta at the cell surface. Found in all tissues; most abundant in placenta and least abundant in brain and heart.

Protein type: EC 2.7.11.30; Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Receptor, misc.; STKR family; TKL group; Type1 subfamily

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: cell surface; lipid raft; plasma membrane; receptor complex; tight junction

Molecular Function: ATP binding; growth factor binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; punt binding; receptor signaling protein activity; SMAD binding; transforming growth factor beta binding; transforming growth factor beta receptor activity; transforming growth factor beta receptor activity, type I

Biological Process: activation of MAPKK activity; activin receptor signaling pathway; anterior/posterior pattern formation; artery morphogenesis; cell cycle arrest; cell motility involved in cell locomotion; collagen fibril organization; embryonic cranial skeleton morphogenesis; endothelial cell activation; epithelial to mesenchymal transition; extracellular structure organization and biogenesis; germ cell migration; heart development; in utero embryonic development; kidney development; mesenchymal cell differentiation; negative regulation of chondrocyte differentiation; negative regulation of transforming growth factor beta receptor signaling pathway; neuron fate commitment; palate development; parathyroid gland development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; pharyngeal system development; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of stress fiber formation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cardiac muscle cell proliferation; regulation of protein ubiquitination; regulation of transcription, DNA-dependent; signal transduction; skeletal development; skeletal morphogenesis; thymus development; transforming growth factor beta receptor signaling pathway; wound healing

Disease: Multiple Self-healing Squamous Epithelioma, Susceptibility To

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