Rabbit Anti-Human Kir4.1 Polyclonal; ATP sensitive inward rectifier potassium channel 10 Antibody; BIRK10 Antibody; KCNJ13 PEN Antibody; Potassium channel inwardly rectifying subfamily J member 10 Antibody; SESAME Antibody inwardly rectifying subfamily J member 10 Antibody; Inward rectifier K(+) channel Kir1.2 Antibody; Potassium channel KCNJ 10 Antibody; ATP-sensitive inward rectifier potassium channel 10 Antibody; Potassium inwardly rectifying channel subfamily J member 10 Antibody; KIR1.2 Antibody; Inwardly rectifying potassium channel Kir1.2 Antibody; ATP-dependent inwardly rectifying potassium channel Kir4.1 Antibody; Glial ATP dependent inwardly rectifying potassium channel KIR4.1 Antibody; Kcnj10 Antibody; KCNJ10 Antibody; IRK10_HUMAN Antibody; KIR4.1 Antibody; Inward rectifier K+ channel KIR1.2 Antibody; ATP dependent inwardly rectifying potassium channel Kir4.1 Antibody; Potassium channel Antibody

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Detects ~42 kDa.

Peptide Affinity Purified

1 mg/ml (lot specific)

Store at -20 degree C.

Small volumes of anti-Kir4.1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Antibodies; Neuroscience; Neurotransmission; Ion Channels; Potassium Channels; Inward-Rectifying Potassium Channels

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

WB: 1:1000
ICC/IF: 1:100

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir4.1 Antibody. (D) Composite.

Western blot analysis of Rat Liver cell lysates showing detection of ~42.5 kDa Kir4.1 protein using Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (SPC-700) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42.5 kDa.

42,508 Da

potassium voltage-gated channel subfamily J member 10

ATP-sensitive inward rectifier potassium channel 10

ATP-sensitive inward rectifier potassium channel 10

This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (). In the kidney, together with KCNJ16, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules.

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