Product Name
Myosin Light Chain 2, aa1-166, His-Tag (MYL2), Recombinant Protein
Full Product Name
Myosin Light Chain 2, aa1-166, His-Tag, Recombinant, Human (MYL2, MLC2, CMH10)
Product Synonym Names
Anti -Myosin Light Chain 2, aa1-166, His-Tag, Recombinant, Human (MYL2, MLC2, CMH10)
Product Gene Name
MYL2 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 12; NC_000012.11 (111348623..111358404, complement). Location: 12q24.11
3D Structure
ModBase 3D Structure for P10916
Purity/Purification
Highly Purified
95% by SDS PAGE. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Form/Format
Supplied as a liquid in 20mM Tris pH 8.0, 20% glycerol, 5mM CaCl2.
Endotoxin Level
1.0 EU/ug of protein (determined by LAL method)
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of MYL2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
MYL2 recombinant protein
Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
Product Categories/Family for MYL2 recombinant protein
Antibodies; Abs to Myosin
NCBI/Uniprot data below describe general gene information for MYL2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000423.2
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NCBI GenBank Nucleotide #
NM_000432.3
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UniProt Primary Accession #
P10916
[Other Products]
UniProt Secondary Accession #
Q16123[Other Products]
UniProt Related Accession #
P10916[Other Products]
Molecular Weight
20.9kD[Similar Products]
NCBI Official Full Name
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Official Synonym Full Names
myosin, light chain 2, regulatory, cardiac, slow
NCBI Official Symbol
MYL2??[Similar Products]
NCBI Official Synonym Symbols
MLC2; CMH10
??[Similar Products]
NCBI Protein Information
myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow
UniProt Protein Name
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Protein Family
Myosin regulatory
UniProt Gene Name
MYL2??[Similar Products]
UniProt Synonym Gene Names
MLC-2; MLC-2v??[Similar Products]
UniProt Entry Name
MLRV_HUMAN
NCBI Summary for MYL2
Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Comments for MYL2
MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.
Protein type: Contractile
Chromosomal Location of Human Ortholog: 12q24.11
Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton
Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding
Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; cardiac muscle contraction; muscle filament sliding; post-embryonic development
Disease: Cardiomyopathy, Familial Hypertrophic, 10
Research Articles on MYL2
1. study concludes the mutations in the last exon of MYL2 are responsible for a novel autosomal recessive lethal myosinopathy due to defects changing the C-terminal tail of the ventricular form of the myosin regulatory light chain
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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