Anti -Myosin Light Chain 2, Phosphorylated (Thr18/Ser19)

For Research Use Only. Not for use in diagnostic procedures.

Chromosome: 12; NC_000012.11 (111348623..111358404, complement). Location: 12q24.11

Polyclonal

IgG

Rabbit

Detects endogenous levels of Thr18 and Ser19 phosphorylated myosin light chain 2 (smooth muscle). Does not crossreact with the cardiac isoform of myosin light chain 2. Species Crossreactivity:

Affinity Purified
Purified by protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). The phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fiber (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2, and Ser9 by PKC and cdc2, is also reported, which inhibits myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Lack of phosphorylation sites on cardiac muscle isoform shows morphological and functional abnormalities on transgenic mice (6).

Antibodies; Abs to Myosin

Western Blot (WB)

Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000

18,789 Da[Similar Products]

myosin, light chain 2, regulatory, cardiac, slow

myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v; RLC of myosin; myosin light chain 2; regulatory light chain of myosin; cardiac ventricular myosin light chain 2; slow cardiac myosin regulatory light chain 2; myosin, light polypeptide 2, regulatory, cardiac, slow

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

MLRV_HUMAN

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq]

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; cytosol; myosin complex; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; muscle filament sliding; cardiac muscle contraction; post-embryonic development

Disease: Cardiomyopathy, Familial Hypertrophic, 10

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