For Research Use Only. Not for use in diagnostic procedures.

Store at 4 degree C for 6 months.

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Small volumes of CD40 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS846753 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the CD40 Ligand, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CD40. The ELISA analytical biochemical technique of the MBS846753 kit is based on CD40 antibody-CD40 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CD40 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CD40. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Background/Introduction: Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. QuickDetectTM CD40 Ligand ELISA kit is a sandwich ELISA assay for measuring the quantity of human CD40 Ligand in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

29,274 Da

CD40 ligand

CD40 ligand

CD40 ligand

CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq26.3

Cellular Component: cell surface; integral to plasma membrane; plasma membrane

Molecular Function: CD40 receptor binding; protein binding

Biological Process: activation of JNK activity; activation of NF-kappaB transcription factor; B cell proliferation; inflammatory response; isotype switching; negative regulation of apoptosis; platelet activation; positive regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of interleukin-4 production; positive regulation of T cell proliferation; regulation of immune response; T cell costimulation; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency With Hyper-igm, Type 1

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