CD154 antibody (YMF323.6.2)

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2a

YMF323.6.2

Rat

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Lyophilized

Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

Small volumes of anti-CD154 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rat anti Human CD154 antibody, clone YMF323.6.2 recognizes the human CD154 cell surface antigen, a 32kDa glycoprotein also known as CD40 ligand. CD154 is expressed on activated T lymphocytes, predominantly CD4+ve cells and also on some basophils and mast cells. Rat anti Human CD154 antibody, clone YMF323.6.2 also recognizes the soluble form of CD154.

Flow Cytometry (FC/FACS)

Figure A. Unstimulated cells stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 and PE conjugated Mouse anti Human CD154 . Figure B. Cells stimulated with PMA and ionomycin for 5 hr then stained with Alexa Fluor 700 conjugated Mouse anti Human CD3 and PE conjugated Mouse anti Human CD154 . All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Recombinant Human CD154 detected with Rat anti Human CD154

CD40 ligand

CD40 ligand

CD40 ligand

CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]

CD40L_HUMAN

The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]

CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; immunoglobulin secretion; B cell differentiation; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1

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