Product Name
CD154 (CD40LG), Recombinant Protein
Full Product Name
CD154, Recombinant, Human (CD40 Ligand, CD40-L, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, T-cell Antigen GP39, TNF-related Activation Protein, TRAP, Tumor Necrosis Factor Ligand Superfamily Member 5, TNF Ligand Superfamily Member 5, TNFSF5)
Product Gene Name
CD40LG recombinant protein
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: X; NC_000023.10 (135730281..135742549). Location: Xq26
3D Structure
ModBase 3D Structure for P29965
Purity/Purification
Highly Purified
~98% by SDS PAGE and HPLC analysis. Endotoxin: 1EU/ug.
Form/Format
Supplied as a lyophilized powde in 10mM sodium phosphate pH7.5. Reconstitute with 10ul 5-10mM sodium phosphate pH7.5.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of CD40LG recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for CD40LG recombinant protein
Molecular Biology; MB-CD Markers
Applications Tested/Suitable for CD40LG recombinant protein
Western Blot (WB)
Application Notes for CD40LG recombinant protein
Suitable for use in Functional Assays and Western Blot.
Dilution: Western Blot: 0.05-0.5ug
NCBI/Uniprot data below describe general gene information for CD40LG. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000065.1
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NCBI GenBank Nucleotide #
NM_000074.2
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UniProt Primary Accession #
P29965
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UniProt Related Accession #
P29965[Other Products]
Molecular Weight
16.3kD [Similar Products]
NCBI Official Full Name
CD40 ligand
NCBI Official Synonym Full Names
CD40 ligand
NCBI Official Symbol
CD40LG??[Similar Products]
NCBI Official Synonym Symbols
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
??[Similar Products]
NCBI Protein Information
CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
UniProt Protein Name
CD40 ligand
UniProt Synonym Protein Names
T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5
Protein Family
CD40 ligand
UniProt Gene Name
CD40LG??[Similar Products]
UniProt Synonym Gene Names
CD40L; TNFSF5; TRAP; CD40-L; TRAP??[Similar Products]
UniProt Entry Name
CD40L_HUMAN
NCBI Summary for CD40LG
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
UniProt Comments for CD40LG
CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
Protein type: Cell adhesion; Membrane protein, integral
Chromosomal Location of Human Ortholog: Xq26
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane
Molecular Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding
Biological Process: B cell proliferation; platelet activation; leukocyte adhesion; regulation of immune response; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis
Disease: Immunodeficiency With Hyper-igm, Type 1
Research Articles on CD40LG
1. A major part of central and effector memory CD8(+) T cells expresses CD40L, one key molecule for CD4(+) T-cell-mediated help.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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