Product Name
3 beta-hydroxysteroid dehydrogenase type 7 (Hsd3b7), Recombinant Protein
Full Product Name
Recombinant Rat 3 beta-hydroxysteroid dehydrogenase type 7 (Hsd3b7)
Product Gene Name
Hsd3b7 recombinant protein
[Similar Products]
Product Synonym Gene Name
Cca2[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-338aa; Full length protein
Sequence
MADSAQVPAL VYLVTGGCGF LGEHIVRMLL EWEPRLRELR VFDLHLSSWL EELKTGPVQV TAIQGDVTQA HEVAAAMAGS HVVIHTAGLV DVFGKASPET IHKVNVQGTQ NVIDACVQTG TRLLVYTSSM EVVGPNVKGH PFYRGNEDTP YEAIHRHPYP CSKALAEQLV LEANGRKGLR FGGRLFRAIP ASVEHGRVYV GNVAWMHILV ARELEQRAAL MGGQVYFCYD KSPYKSYEDF NMEFLSPCGL RLIGTHPLLP YWLLVLLTAL NALLQWLLRP LVLYTPLLNP YTLAVANTTF TVSTNKAQRH FGYKPLFSWE ESRARTIHWV QAMEGSAW
3D Structure
ModBase 3D Structure for O35048
Host
Cell Free Expression
Form/Format
Liquid containing glycerol
Storage Buffer
Tris-based buffer, 50% glycerol.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Hsd3b7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for Hsd3b7 recombinant protein
Transmembrane Protein
Application Notes for Hsd3b7 recombinant protein
This is a recombinant transmembrane protein expressed in a cell-free expression system.
NCBI/Uniprot data below describe general gene information for Hsd3b7. It may not necessarily be applicable to this product.
NCBI Accession #
NP_647545.1
[Other Products]
NCBI GenBank Nucleotide #
NM_139329.1
[Other Products]
UniProt Primary Accession #
O35048
[Other Products]
UniProt Related Accession #
O35048[Other Products]
Molecular Weight
37,781 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase type 7
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
NCBI Official Symbol
Hsd3b7??[Similar Products]
NCBI Official Synonym Symbols
Cca2
??[Similar Products]
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase type 7
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase type 7
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase (EC:1.1.1.-); C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase (EC:1.1.1.181); Confluent 3Y1 cell-associated 2
Protein Family
3 beta-hydroxysteroid dehydrogenase
UniProt Gene Name
Hsd3b7??[Similar Products]
UniProt Synonym Gene Names
3-beta-HSD VII; C(27) 3-beta-HSD??[Similar Products]
UniProt Entry Name
3BHS7_RAT
NCBI Summary for Hsd3b7
protein that is expressed in under growth-arrest conditions [RGD, Feb 2006]
UniProt Comments for Hsd3b7
HSD3B7: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1); also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis. Belongs to the 3-beta-HSD family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Membrane protein, multi-pass; EC 1.1.1.181
Cellular Component: endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle
Molecular Function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity; cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity; isomerase activity
Biological Process: bile acid metabolic process; cholesterol catabolic process; regulation of cell growth; steroid biosynthetic process
Research Articles on Hsd3b7
1. results suggest that the mechanisms of regulation of these bile acid metabolizing enzymes are significantly different during liver regeneration
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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