Product Name
3 beta-hydroxysteroid dehydrogenase type 7 (Hsd3b7), Recombinant Protein
Full Product Name
Recombinant Mouse 3 beta-hydroxysteroid dehydrogenase type 7 (Hsd3b7)
Product Gene Name
Hsd3b7 recombinant protein
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Sequence Positions
1-369aa; full length protein
Sequence
MADSAQVPTL VYLVTGGCGF LGEHIVRMLL EREPRLRELR VFDLHLSSWL EELKAGPVQV TAIQGDVTQA HEVAAAMSGS HVVIHTAGLV DVFGKASPKT IHKVNVQGTQ NVIDACVQTG TQYLVYTSSM EVVGPNIKGH PFYRGNEDTP YEAVHSHPYP CSKALAEQLV LEANGRKVNG GLPLVTCALR PTGIYGEGHQ VMRDFYYQGL RFGGRLFRAV PASVEHGRVY VGNVAWMHIL VARELEQRAA LMGGQVYFCY DKSPYKSYED FNMEFLSPCG LRLIGAHPLL PYWLLVLLAT LNALLQWLLR PLVLYTPLLN PYTLAMANTT FTVSTNKAQR HFGYKPLFSW EESRTRTIQW VQAMEGSAR
3D Structure
ModBase 3D Structure for Q9EQC1
Host
Cell Free Expression
Form/Format
Liquid containing glycerol
Storage Buffer
Tris-based buffer, 50% glycerol.
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Other Notes
Small volumes of Hsd3b7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for Hsd3b7 recombinant protein
Transmembrane Protein
Application Notes for Hsd3b7 recombinant protein
This is a recombinant transmembrane protein expressed in a cell-free expression system.
NCBI/Uniprot data below describe general gene information for Hsd3b7. It may not necessarily be applicable to this product.
NCBI Accession #
NP_598704.2
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NCBI GenBank Nucleotide #
NM_133943.2
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UniProt Primary Accession #
Q9EQC1
[Other Products]
UniProt Secondary Accession #
A2RTR5[Other Products]
UniProt Related Accession #
Q9EQC1[Other Products]
Molecular Weight
41,135 Da
NCBI Official Full Name
3 beta-hydroxysteroid dehydrogenase type 7 isoform a
NCBI Official Synonym Full Names
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
NCBI Official Symbol
Hsd3b7??[Similar Products]
NCBI Official Synonym Symbols
AI195443; BB098564
??[Similar Products]
NCBI Protein Information
3 beta-hydroxysteroid dehydrogenase type 7
UniProt Protein Name
3 beta-hydroxysteroid dehydrogenase type 7
UniProt Synonym Protein Names
3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase (EC:1.1.1.-); C(27) 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase (EC:1.1.1.181)
Protein Family
3 beta-hydroxysteroid dehydrogenase
UniProt Gene Name
Hsd3b7??[Similar Products]
UniProt Synonym Gene Names
3-beta-HSD VII; C(27) 3-beta-HSD??[Similar Products]
UniProt Entry Name
3BHS7_MOUSE
UniProt Comments for Hsd3b7
HSD3B7: The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Defects in HSD3B7 are the cause of congenital bile acid synthesis defect type 1 (CBAS1); also known as neonatal progressive intrahepatic cholestasis. CBAS1 is due to a primary defect in bile synthesis leading to progressive liver disease. Clinical features include neonatal jaundice, severe intrahepatic cholestasis and cirrhosis. Belongs to the 3-beta-HSD family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Lipid Metabolism - primary bile acid biosynthesis; Membrane protein, multi-pass; EC 1.1.1.181; Oxidoreductase
Cellular Component: endoplasmic reticulum; integral to membrane; intracellular membrane-bound organelle; membrane
Molecular Function: (R)-2-hydroxyglutarate dehydrogenase activity; (R)-2-hydroxyisocaproate dehydrogenase activity; 2-hydroxytetrahydrofuran dehydrogenase activity; 3-beta-hydroxy-delta5-steroid dehydrogenase activity; 3-ketoglucose-reductase activity; 5-exo-hydroxycamphor dehydrogenase activity; acetoin dehydrogenase activity; aldo-keto reductase activity; arabinose reductase activity; C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity; cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase activity; D-arabinitol dehydrogenase (NADP+) activity; epoxide dehydrogenase activity; gluconate dehydrogenase activity; isocitrate dehydrogenase activity; mevaldate reductase activity; oxidoreductase activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; phenylcoumaran benzylic ether reductase activity; steroid dehydrogenase activity; steroid dehydrogenase activity, acting on the CH-CH group of donors; steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; xylose reductase activity
Biological Process: cholesterol catabolic process; regulation of cell growth; steroid biosynthetic process
Research Articles on Hsd3b7
1. Findings indicate that CYP7B1 and HSD3B7, as well as CH25H, have essential roles in controlling oxysterol production in lymphoid tissues.
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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