Product Name
Myosin Light Chain 2 (MLC2), Polyclonal Antibody
Full Product Name
Anti-Myosin Light Chain 2 Rabbit pAb
Product Synonym Names
MYL2; Myosin light chain 2; MLC-2; RLC of myosin; CMH10; MLC-2v
Product Gene Name
anti-MLC2 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P10916
Species Reactivity
Human, Mouse, Rat
Specificity
Myosin Light Chain 2 Antibody detects endogenous levels of Myosin Light Chain 2
Purity/Purification
Affinity Chromatography
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunogen
A synthesized peptide derived from human Myosin Light Chain 2
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Other Notes
Small volumes of anti-MLC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MLC2 antibody
Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner.
Applications Tested/Suitable for anti-MLC2 antibody
Western Blot (WB), Immunhistochemistry (IHC), Immunoprecipitation (IP)
Application Notes for anti-MLC2 antibody
WB: 1:1000-1:5000
IHC: 1:50-1:200
IP: 1:50
Western Blot (WB) of anti-MLC2 antibody
Western blot analysis of Myosin Light Chain 2 expression in Mouse heart lysate.
Immunohistochemistry (IHC) of anti-MLC2 antibody
Immunohistochemical analysis of paraffin-embedded human heart, using Myosin Light Chain 2 Antibody.
NCBI/Uniprot data below describe general gene information for MLC2. It may not necessarily be applicable to this product.
NCBI Accession #
NP_000423.2
[Other Products]
NCBI GenBank Nucleotide #
NM_000432.3
[Other Products]
UniProt Primary Accession #
P10916
[Other Products]
UniProt Secondary Accession #
Q16123[Other Products]
UniProt Related Accession #
P10916[Other Products]
NCBI Official Full Name
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NCBI Official Synonym Full Names
myosin light chain 2
NCBI Official Symbol
MYL2??[Similar Products]
NCBI Official Synonym Symbols
MLC2; CMH10; MLC-2s/v
??[Similar Products]
NCBI Protein Information
myosin regulatory light chain 2, ventricular/cardiac muscle isoform
UniProt Protein Name
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
UniProt Synonym Protein Names
Cardiac myosin light chain 2
UniProt Gene Name
MYL2??[Similar Products]
UniProt Synonym Gene Names
MLC-2s/v??[Similar Products]
NCBI Summary for MLC2
Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Comments for MLC2
Contractile protein that plays a role in heart development and function (). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin s to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly ().
Research Articles on MLC2
1. A on its own is incapable of triggering clinical HCM in most carriers. However, the presence of an additional risk factor for hypertrophy, particularly hypertension, adds to the development of HCM.">The results show that the MYL2 mutation c.64G > A on its own is incapable of triggering clinical HCM in most carriers. However, the presence of an additional risk factor for hypertrophy, particularly hypertension, adds to the development of HCM.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
