Slow cardiac myosin regulatory light chain 2; MLC2; CMH10; DKFZp779C0562; Slow cardiac myosin regulatory light chain 2; MYL2; Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2; MLC 2v; MYL 2; Cardiac ventricular myosin light chain 2; RLC of myosin; Myosin light chain 2 regulatory cardiac slow; Myosin light polypeptide 2 regulatory cardiac slow; Myosin regulatory light chain 2 ventricular cardiac muscle isoform; Myosin regulatory light chain 2 ventricular/cardiac muscle isoform; Regulatory light chain of myosin

For Research Use Only. Not for use in diagnostic procedures.

Monoclonal

IgG2b, k

AT3B2

Mouse

By protein-G affinity chromatography

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

1mg/ml (lot specific)

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Cytoskeleton

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

The extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of MYL2 in A431 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MYL2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

18,789 Da

myosin light chain 2

myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

MLC-2s/v??[Similar Products]

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Contractile protein that plays a role in heart development and function (). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin s to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly ().

Macera MJ, et al., (1992) Genomics. 13 (3):829-31; Poetter K, et al., (1996) Nat Genet. 13 (1):63-9

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