Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2v

For Research Use Only. Not for use in diagnostic procedures.

Polyclonal

Rabbit

Recognizes endogenous levels of MYL2 protein.

The antibody was purified by immunogen affinity chromatography.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Small volumes of anti-MYL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Rabbit polyclonal antibody to MYL2

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

WB: 1/500 - 1/2000; IF/IC: 1/50 - 1/100

Western blot analysis of MYL2 expression in MCF7 (A), mouse skeletal muscle (B) whole cell lysates.

Immunofluorescent analysis of MYL2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

18,789 Da

myosin light chain 2

myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

MLC-2s/v??[Similar Products]

MLRV_HUMAN

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: actin cytoskeleton; cytoskeleton; cytosol; myosin complex; sarcomere

Molecular Function: actin monomer binding; calcium ion binding; protein binding

Biological Process: cardiac myofibril assembly; heart contraction; heart development; muscle filament sliding; negative regulation of cell growth; regulation of striated muscle contraction; regulation of the force of heart contraction; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Familial Hypertrophic, 10

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