Full Product Name
PHF8 polyclonal antibody
Product Synonym Names
JHDM1F; MRXSSD; ZNF422
Product Gene Name
anti-PHF8 antibody
[Similar Products]
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UPP1
Purity/Purification
Affinity Purified
Form/Format
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
Appearance: Colorless liquid
Immunogen
Polyclonal antibody raised in rabbit against human PHF8 (PHD finger protein 8), using 3 different KLH-conjugated synthetic peptides.
Positive Control
Western blot: mouse embryonic stem cells, ELISA: Peptides.
Handling
The antibody solution should be gently mixed before use.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Other Notes
Small volumes of anti-PHF8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PHF8 antibody
Polyclonal Antibody to detect PHF8 in human samples.
Background: PHD finger protein 8 is a Jumonji domain containing protein. Like other members of the jumonji family, PHF8 may therefore play a role in histone demethylation. Mutations in PHF8 lead to Siderius type X-linked mental retardation (MRXSSD), a mild to borderline type of mental retardation.
Product Categories/Family for anti-PHF8 antibody
Epigenetics; Eraser Enzymes; Histone Demethylases (HDMs); Antibodies & Blocking PeptdiesAntibodies & Supporting Tools; Antibodies for Epigenetics ResearchAntibodies & Supporting Tools; Primary Antibodies (A-Z)
Applications Tested/Suitable for anti-PHF8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-PHF8 antibody
Western Blot: 1:1000, ELISA: 1:100 - 1:500.
Testing Data of anti-PHF8 antibody
NCBI/Uniprot data below describe general gene information for PHF8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001171825.1
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NCBI GenBank Nucleotide #
NM_001184896.1
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UniProt Primary Accession #
Q9UPP1
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UniProt Secondary Accession #
Q5H9U5; Q5JPR9; Q5JPS0; Q5JPS2; Q5JPS3; Q5VUJ4; Q7Z6D4; Q9HAH2; B3KMV4; B7Z911[Other Products]
UniProt Related Accession #
Q9UPP1[Other Products]
Molecular Weight
98,290 Da
NCBI Official Full Name
histone lysine demethylase PHF8 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 8
NCBI Official Symbol
PHF8??[Similar Products]
NCBI Official Synonym Symbols
JHDM1F; MRXSSD; ZNF422
??[Similar Products]
NCBI Protein Information
histone lysine demethylase PHF8
UniProt Protein Name
Histone lysine demethylase PHF8
UniProt Synonym Protein Names
PHD finger protein 8
Protein Family
Histone lysine demethylase
UniProt Gene Name
PHF8??[Similar Products]
UniProt Synonym Gene Names
KIAA1111; ZNF422??[Similar Products]
UniProt Entry Name
PHF8_HUMAN
NCBI Summary for PHF8
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
UniProt Comments for PHF8
PHF8: a PHD finger protein with N(epsilon)-methyl lysine histone demethylase activity. Associated with mental retardation and cleft lip/palate. Its active site is closely related to those of FBXL10 and 11, all of which are selective for di- and mono-methylated lysine. In contrast, the JMJD2 demethylases are selective for tri- and di-methylated lysine.
Protein type: EC 1.14.11.27; Demethylase; Nucleolus
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nucleus
Molecular Function: protein binding; histone demethylase activity; zinc ion binding; iron ion binding; histone demethylase activity (H3-K36 specific); oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; histone demethylase activity (H3-K9 specific); methylated histone residue binding
Biological Process: establishment and/or maintenance of chromatin architecture; histone H3-K9 demethylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; mitotic cell cycle; brain development; G1/S transition of mitotic cell cycle; positive regulation of transcription from RNA polymerase I promoter
Disease: Siderius X-linked Mental Retardation Syndrome
Research Articles on PHF8
1. PHF2 is likely to repress rDNA transcription by competing with PHF8 for binding of ribosomal DNA promoter and by recruiting H3K9me2/3 methyltransferase SUV39H1.
Precautions
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Disclaimer
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