Full Product Name
PHF8 siRNA (Human)
Product Synonym Names
KIAA1111; ZNF422; Histone lysine demethylase PHF8; PHD finger protein 8
Product Gene Name
PHF8 sirna
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Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UPP1
Specificity
PHF8 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Purity/Purification
> 97%
Form/Format
Lyophilized powder
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PHF8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Other Notes
Small volumes of PHF8 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
PHF8 sirna
siRNA to inhibit PHF8 expression using RNA interference
Applications Tested/Suitable for PHF8 sirna
RNA Interference (RNAi)
NCBI/Uniprot data below describe general gene information for PHF8. It may not necessarily be applicable to this product.
NCBI Accession #
NP_001171825.1
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NCBI GenBank Nucleotide #
NM_001184896.1
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UniProt Primary Accession #
Q9UPP1
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UniProt Secondary Accession #
Q5H9U5; Q5JPR9; Q5JPS0; Q5JPS2; Q5JPS3; Q5VUJ4; Q7Z6D4; Q9HAH2; B3KMV4; B7Z911[Other Products]
UniProt Related Accession #
Q9UPP1[Other Products]
Molecular Weight
98,290 Da
NCBI Official Full Name
histone lysine demethylase PHF8 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 8
NCBI Official Symbol
PHF8??[Similar Products]
NCBI Official Synonym Symbols
JHDM1F; MRXSSD; ZNF422
??[Similar Products]
NCBI Protein Information
histone lysine demethylase PHF8
UniProt Protein Name
Histone lysine demethylase PHF8
UniProt Synonym Protein Names
PHD finger protein 8
Protein Family
Histone lysine demethylase
UniProt Gene Name
PHF8??[Similar Products]
UniProt Synonym Gene Names
KIAA1111; ZNF422??[Similar Products]
UniProt Entry Name
PHF8_HUMAN
NCBI Summary for PHF8
The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
UniProt Comments for PHF8
PHF8: a PHD finger protein with N(epsilon)-methyl lysine histone demethylase activity. Associated with mental retardation and cleft lip/palate. Its active site is closely related to those of FBXL10 and 11, all of which are selective for di- and mono-methylated lysine. In contrast, the JMJD2 demethylases are selective for tri- and di-methylated lysine.
Protein type: EC 1.14.11.27; Demethylase; Nucleolus
Chromosomal Location of Human Ortholog: Xp11.22
Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nucleus
Molecular Function: protein binding; histone demethylase activity; zinc ion binding; iron ion binding; histone demethylase activity (H3-K36 specific); oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; chromatin binding; histone demethylase activity (H3-K9 specific); methylated histone residue binding
Biological Process: establishment and/or maintenance of chromatin architecture; histone H3-K9 demethylation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; brain development; mitotic cell cycle; G1/S transition of mitotic cell cycle; positive regulation of transcription from RNA polymerase I promoter
Disease: Siderius X-linked Mental Retardation Syndrome
Research Articles on PHF8
1. PHF2 is likely to repress rDNA transcription by competing with PHF8 for binding of ribosomal DNA promoter and by recruiting H3K9me2/3 methyltransferase SUV39H1.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
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